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Glyco-Engineering pp 73–90Cite as

Progress in Yeast Glycosylation Engineering

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1321))

Abstract

While yeast are lower eukaryotic organisms, they share many common features and biological processes with higher eukaryotes. As such, yeasts have been used as model organisms to facilitate our understanding of such features and processes. To this end, a large number of powerful genetic tools have been developed to investigate and manipulate these organisms. Going hand-in-hand with these genetic tools is the ability to efficiently scale up the fermentation of these organisms, thus making them attractive hosts for the production of recombinant proteins. A key feature of producing recombinant proteins in yeast is that these proteins can be readily secreted into the culture supernatant, simplifying any downstream processing. A consequence of this secretion is that the proteins typically pass through the secretory pathway, during which they may be exposed to various posttranslational modifications. The addition of glycans is one such modification. Unfortunately, while certain aspects of glycosylation are shared between lower and higher eukaryotes, significant differences exist. Over the last two decades much research has focused on engineering the glycosylation pathways of yeast to more closely resemble those of higher eukaryotes, particularly those of humans for the production of therapeutic proteins. In the current review we shall highlight some of the key achievements in yeast glyco-engineering which have led to humanization of both the N- and O-linked glycosylation pathways.

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Authors and Affiliations

  1. GlycoFi (A Wholly Owned Subsidiary of Merck & Co., Inc.), Biologics Discovery, Merck Research Laboratories, Lebanon, NH, USA

    Stephen R. Hamilton

  2. Institute for Applied Cancer Science, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Dongxing Zha

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  1. Stephen R. Hamilton
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  1. University of Natural Resources and Life Sciences, Vienna, Austria

    Alexandra Castilho

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Hamilton, S.R., Zha, D. (2015). Progress in Yeast Glycosylation Engineering. In: Castilho, A. (eds) Glyco-Engineering. Methods in Molecular Biology, vol 1321. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2760-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2760-9_6

  • Publisher Name: Humana Press, New York, NY

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