Mass Spectrometric Analysis of Oligo- and Polysialic Acids

  • Christina E. Galuska
  • Kai Maass
  • Sebastian P. GaluskaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1321)


Oligo- and polysialic acids (oligo/polySia) are involved in numerous biological processes depending on the chain length, the comprised type of sialic acids, as well as the glycosidic linkages. Here, we describe the determination of the composition, the sequence, as well as the linkages between the sialic acid residues of lactonized oligo/polySia using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)(/MS) and electrospray-ionization (ESI)-MS(n).

Key words

Polysialic acid DMB-derivatization Linkage analysis HPLC MALDI-TOF-mass spectrometry Electrospray-ion trap-mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Christina E. Galuska
    • 1
  • Kai Maass
    • 2
  • Sebastian P. Galuska
    • 1
    Email author
  1. 1.Institute of Biochemistry, Faculty of MedicineUniversity of GiessenGiessenGermany
  2. 2.Institute for Organic Chemistry, Faculty of Biology and ChemistryUniversity of GiessenGiessenGermany

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