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N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements

  • Herta Steinkellner
  • Alexandra Castilho
Part of the Methods in Molecular Biology book series (MIMB, volume 1321)

Abstract

Plants are being developed as a cost-effective production system for biopharmaceuticals in large quantities. Although plants properly fold and assemble complex proteins from human origin, one issue that needs to be addressed is their glycan structure. In the past years we have been witnessing outstanding results in targeted manipulation of the plant N-glycosylation pathway allowing recombinant proteins to be produced with human-type oligosaccharides at large homogeneity. This opens new possibility in manufacturing next-generation biopharmaceuticals.

This review presents a variety of technologies and strategies that are being employed to engineer the plant N-glycosylation, thus pointing to the enormous potential of plants being used as a novel production system with unique features and possibilities.

Key words

Glyco-engineering Plants Recombinant protein expression Glycosyltransferases Glycosidases Subcellular targeting 

Abbreviations

C1GalT1

Drosophila melanogaster Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1

CMAS

Human CMP-N-acetylneuraminic acid synthase

CST

Mouse CMP-sialic acid transporter

CTS

Cytoplasmic tail, transmembrane domain, and stem

FUT11

A. thaliana α1,3-fucosyltransferase

FUT8

Human α1,6-fucosyltransferase

FUT9a

Human α1,3-fucosyltransferase IXa

GalNAc-T2

Human polypeptide N-acetylgalactosaminyltransferase

GalT

Human β1,4-galactosyltransferase

GE

Pseudomonas aeruginosa or Yersinia enterocolitica GMI, Golgi mannosidase I

GMII

Golgi mannosidase II

GNE

Mouse UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine-kinase

GnT-I

α1,3-Mannosyl-β1,2-N-acetylglucosaminyltransferase I

GnT-II

α1,6-Mannosyl-β1,2-N-acetylglucosaminyltransferase II

GnT-III

Human β1,4-mannosyl-β1,4-N-acetylglucosaminyltransferase III

GnT-IV

Human α1,3-mannosyl-β1,4-N-acetylglucosaminyltransferase IVa

GnT-V

Human α1,6-mannosyl-β1,6-N-acetylglucosaminyltransferase V

HEXO1 and HEXO3

Arabidopsis β-N-acetylhexosaminidases 1 and 3

NANS

Human N-acetylneuraminic acid phosphate-synthase

P4H

Prolyl-4-hydroxylase

ST

Rat or human α2,6-sialyltransferase

ST3GalI

Human α2,3-sialyltransferase

ST6GalNAc4

Mouse α2,6-sialyltransferase

UDP-GlcNAc/UDP-GalNAc transporter

UPD-GlcNAc 4-epimerase

UGT

Caenorhabditis elegans

XylT

β1,2-Xylosyltransferase

α1,4-FucT

α1,4-Fucosyltransferase

β1,3-GalT

β1,3-Galactosyltransferase

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Herta Steinkellner
    • 1
  • Alexandra Castilho
    • 1
  1. 1.Department of Applied Genetics and Cell BiologyUniversity of Natural Resources and Life SciencesViennaAustria

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