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SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation

  • Dieter Palmberger
  • Dubravko Rendic
Part of the Methods in Molecular Biology book series (MIMB, volume 1321)

Abstract

Observed different glycosylation patterns of glycoconjugates (recombinantly) produced in various eukaryotic organisms are a direct consequence of differences in numerous proteins involved in biosynthesis of the relevant glycan chains in these species. The need for efficient, robust and flexible methods for recombinant expression of proteins is met by the recently described MultiBac technology, an advanced and optimized baculovirus-based system for simultaneous recombinant protein expression in insect cells. A derivative of MultiBac technology, the SweetBac system aims at the modification of the glycosylation potential of insect cells as expression hosts. The application of SweetBac, including the methods needed to investigate the glycosylation pattern of the purified recombinant protein, is described in this chapter.

Key words

SweetBac MultiBac Baculovirus Insect cells Recombinant 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Dieter Palmberger
    • 1
  • Dubravko Rendic
    • 2
  1. 1.Department of BiotechnologyUniversity of Natural Resources and Life SciencesViennaAustria
  2. 2.Department of ChemistryUniversity of Natural Resources and Life SciencesViennaAustria

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