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An Overview and History of Glyco-Engineering in Insect Expression Systems

  • Christoph Geisler
  • Hideaki Mabashi-Asazuma
  • Donald L. Jarvis
Part of the Methods in Molecular Biology book series (MIMB, volume 1321)

Abstract

Insect systems, including the baculovirus-insect cell and Drosophila S2 cell systems are widely used as recombinant protein production platforms. Historically, however, no insect-based system has been able to produce glycoproteins with human-type glycans, which often influence the clinical efficacy of therapeutic glycoproteins and the overall structures and functions of other recombinant glycoprotein products. In addition, some insect cell systems produce N-glycans with immunogenic epitopes. Over the past 20 years, these problems have been addressed by efforts to glyco-engineer insect-based expression systems. These efforts have focused on introducing the capacity to produce complex-type, terminally sialylated N-glycans and eliminating the capacity to produce immunogenic N-glycans. Various glyco-engineering approaches have included genetically engineering insect cells, baculoviral vectors, and/or insects with heterologous genes encoding the enzymes required to produce various glycosyltransferases, sugars, nucleotide sugars, and nucleotide sugar transporters, as well as an enzyme that can deplete GDP-fucose. In this chapter, we present an overview and history of glyco-engineering in insect expression systems as a prelude to subsequent chapters, which will highlight various methods used for this purpose.

Key words

Insect cells Baculovirus Baculovirus insect cell system Drosophila expression system Glyco-engineering Glycosylation 

Notes

Acknowledgements

Research on insect protein glycosylation pathways, the baculovirus-insect cell system, and insect expression system engineering in the authors’ labs at the University of Wyoming and GlycoBac is currently supported by National Institute of General Medical Sciences grants R44GM093411, R43GM102982, R43GM109504, and National Institute of Allergy and Infectious Diseases grant R43AI112118. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences, the National Institute of Allergy and Infectious Disease, or the National Institutes of Health.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Christoph Geisler
    • 1
  • Hideaki Mabashi-Asazuma
    • 2
  • Donald L. Jarvis
    • 1
    • 2
  1. 1.GlycoBac, LLCLaramieUSA
  2. 2.Department of Molecular BiologyUniversity of WyomingLaramieUSA

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