Abstract
In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fredriksson S, Gullberg M, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM et al (2002) Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol 20:473–477
Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C et al (2004) Cytokine detection by antibody-based proximity ligation. Proc Natl Acad Sci U S A 101:8420–8424
Soderberg O, Gullberg M, Jarvius M, Ridderstrale K, Leuchowius KJ, Jarvius J et al (2006) Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat Methods 3:995–1000
Blokzijl A, Friedman M, Ponten F, Landegren U (2010) Profiling protein expression and interactions: proximity ligation as a tool for personalized medicine. J Intern Med 268:232–245
Tsai CL, Koong AC, Hsu FM, Graber M, Chen IS, Cheng JC (2013) Biomarker studies on radiotherapy to hepatocellular carcinoma. Oncology 84(Suppl 1):64–68
Hofacre CL, Glisson JR, Kleven SH, Brown J, Rowland GN (1989) Evaluation of Pasteurella multocida mutants of low virulence. I. Development and pathogenicity. Avian Dis 33:270–274
Petri MK, Koch P, Stenzinger A, Kuchelmeister K, Nestler U, Paradowska A et al (2011) PTPIP51, a positive modulator of the MAPK/Erk pathway, is upregulated in glioblastoma and interacts with 14-3-3beta and PTP1B in situ. Histol Histopathol 26:1531–1543
Hagglund MG, Hellsten SV, Bagchi S, Ljungdahl A, Nilsson VC, Winnergren S et al (2013) Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system. BMC Neurosci 14:54
Kamali-Moghaddam M, Pettersson FE, Wu D, Englund H, Darmanis S, Lord A et al (2010) Sensitive detection of Abeta protofibrils by proximity ligation–relevance for Alzheimer’s disease. BMC Neurosci 11:124
Latge JP, Bouziane H, Diaquin M (1988) Ultrastructure and composition of the conidial wall of Cladosporium cladosporioides. Can J Microbiol 34:1325–1329
Acknowledgements
The authors thank Prof. Ulf Landegren at Uppsala University for constructive feedback during the preparation of this manuscript. Work by Bagchi and Fredriksson is supported by Swedish Research Council, The Swedish Brain Foundation, The Swedish Society for Medical Research, The Novo Nordisk foundation, ʿÅhléns foundation, Engkvist Foundation, Thurings Foundation for metabolic research, Gunvor and Josef Anérs foundation, and Tore Nilssons foundation, and work by Wallén-Mackenzie by The Swedish Research Council, The Swedish Brain Foundation, Parkinsonfonden, ʿÅhléns Foundation and the Hållsten Research Foundation.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Bagchi, S., Fredriksson, R., Wallén-Mackenzie, Å. (2015). In Situ Proximity Ligation Assay (PLA). In: Hnasko, R. (eds) ELISA. Methods in Molecular Biology, vol 1318. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2742-5_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2742-5_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2741-8
Online ISBN: 978-1-4939-2742-5
eBook Packages: Springer Protocols