Nitrocellulose Membrane: The New Canvas

  • Jasmin R. Kurien
  • Bianca A. Kurien
Part of the Methods in Molecular Biology book series (MIMB, volume 1314)


The use of nitrocellulose membranes for invisible ink-messaging or marking for orientation purposes has been carried out with diluted alkaline phosphatase, followed by development with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate and chemiluminescence detection systems. Here, we show that nitrocellulose membrane can serve as a canvas for art using alkaline phosphatase and NBT/BCIP detection method.

Key words

Nitrocellulose membrane Art Canvas Alkaline phosphatase NBT/BCIP 


  1. 1.
    Kurien BT (2009) Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays. Methods Mol Biol 536:573–581PubMedCrossRefGoogle Scholar
  2. 2.
    Kurien BT, Scofield RH (eds) (2009) Protein blotting and detection: methods and protocols, vol 536, Methods in molecular biology. Springer, New York, USAGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Mustang High SchoolMustangUSA
  2. 2.YukonUSA

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