Abstract
Mitochondria are highly dynamic organelles that undergo fusion and fission on a relatively fast time scale. Here, a straightforward method is described for capturing mitochondrial fusion events in real time using a photoconvertible fluorescent protein and a far-field fluorescence microscope equipped with appropriate image acquisition and analysis software. The Kaede photoconvertible fluorescent protein is tagged with a mitochondrial targeting sequence and delivered to primary neurons by lentiviral transduction, which ensures efficient low copy number transgene insertion, as well as stable transgene expression.
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Owens, G.C., Edelman, D.B. (2015). Photoconvertible Fluorescent Protein-Based Live Imaging of Mitochondrial Fusion. In: Pfannkuche, K. (eds) Cell Fusion. Methods in Molecular Biology, vol 1313. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2703-6_18
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DOI: https://doi.org/10.1007/978-1-4939-2703-6_18
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