Immunohistochemistry Relative to Gravity: A Simple Method to Retain Information About Gravity for Immunolocalization and Histochemistry

Part of the Methods in Molecular Biology book series (MIMB, volume 1309)

Abstract

We describe a simple method to preserve information about a plant organ’s orientation relative to the direction of the gravity vector during sample processing for immunolocalization or histochemical analysis of cell biological processes. This approach has been used in gravity stimulated roots of Arabidopsis thaliana and Zea mays to study PIN3 relocalization, study the asymmetrical remodeling of the actin network and the cortical microtubule array, and to reveal the asymmetrical expression of the auxin signaling reporter DR5::GUS. This method enables the rapid analysis of a large number of samples from a variety of genotypes, as well as from tissue that may be too thick for microscopy in live plants.

Key words

Gravity Gravitropism Plants Immunolocalization Immunohistochemistry Cell Biology Histochemistry Signal Transduction Hormones Auxin 

Notes

Acknowledgement

The authors would like to thank K. Rawlins for critical reading and helpful suggestions.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity of Alaska AnchorageAnchorageUSA
  2. 2.Genetics DepartmentUniversity of WisconsinMadisonUSA

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