Spectrophotometric Methods to Determine Protein Concentration

Part of the Methods in Molecular Biology book series (MIMB, volume 1312)

Abstract

Measuring the concentration of proteins is an essential part of enzyme evaluations or to monitor protein yields during protein isolation procedures. Decisions on the usefulness of any isolation procedure depend on knowing the relative concentrations of a particular protein or enzyme in relation to the concentrations of all the proteins present. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting with the protein. This review describes protein determination methods for measuring protein concentration in solution.

Key words

Protein staining Coomassie Bicinchoninic acid Lowry Absorbance Bradford Protein concentration 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.BiochemistryUniversity of KwaZulu-NatalScottsvilleSouth Africa

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