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Protein Detection by Simple Western™ Analysis

  • Valerie M. Harris
Part of the Methods in Molecular Biology book series (MIMB, volume 1312)

Abstract

Protein Simple© has taken a well-known protein detection method, the western blot, and revolutionized it. The Simple Western™ system uses capillary electrophoresis to identify and quantitate a protein of interest. Protein Simple© provides multiple detection apparatuses (Wes, Sally Sue, or Peggy Sue) that are suggested to save scientists valuable time by allowing the researcher to prepare the protein sample, load it along with necessary antibodies and substrates, and walk away. Within 3–5 h the protein will be separated by size, or charge, immuno-detection of target protein will be accurately quantitated, and results will be immediately made available. Using the Peggy Sue instrument, one study recently examined changes in MAPK signaling proteins in the sex-determining stage of gonadal development. Here the methodology is described.

Key words

Protein Simple© Simple Western™ Protein 

References

  1. 1.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to NC sheets: procedure and applications. Proc Natl Acad Sci U S A 76:4350–4354PubMedCentralPubMedCrossRefGoogle Scholar
  2. 2.
    Erik G, Thayer W, John P (2013) Peggy™: size- or charge-based western blotting at the push of a button. Nature Methods 10:i–iiGoogle Scholar
  3. 3.
    Protein Simple©. “The Simple Western Overview™”. Protein Simple© 2011–2014. www.proteinsimple.com
  4. 4.
    Siggers P, Carre’ G-A, Bogani D, Warr N, Wells S et al (2014) A novel mouse Fgfr2 mutant, hobbyhorse (hob), exhibits complete XY gonadal sex reversal. PLoS One 9(6):e100447PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of PathologyUniversity of Oklahoma Health Science CenterOklahoma CityUSA

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