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B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells

  • Protocol
Western Blotting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

The B-cell ELISPOT assay is a sensitive tool that can be utilized to measure total immunoglobulin (Ig) and antigen-specific antibody-secreting cells. Typically, membrane-bound antigen enables binding of antibody secreted by B-cells. Bound antibody is then detected by using an anti-Ig antibody and a colorimetric substrate, resulting in colored spots on the membrane that can be easily enumerated. Here we have described a method to quantitate antigen-specific antibody-secreting cells from the spleen or bone marrow of a vaccinated mouse.

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Correspondence to Kristi A. Koelsch Ph.D. .

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© 2015 Springer Science+Business Media New York

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Shah, H.B., Koelsch, K.A. (2015). B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_42

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_42

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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