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Western Blotting pp 415–417Cite as

Blotting of Coomassie Blue-Stained Proteins from PAGE Gels to Transparencies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

A simple, convenient, and inexpensive method for long-term non-photographic storage of information present in electrophoresis gel, based on protein blotting patterns, is presented here.

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References

  1. Chu Y, Whitesides GM (1993) A convenient procedure for transfer blotting of Coomassie blue stained proteins from PAGE-gels to transparencies. Biotechniques 14(6):925–930

    CAS  PubMed  Google Scholar 

  2. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage t4. Nature 227:680–685

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  3. Margolis J, Kendrick KG (1968) Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Anal Biochem 25:341–362

    Article  Google Scholar 

  4. Rickwood D, Hames BD (1990) Gel electrophoresis of nucleic acids: a practical approach, 2nd edn. IRL Press, Oxford

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Author information

Authors and Affiliations

  1. Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA

    Jinny Paul

Authors
  1. Jinny Paul
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Corresponding author

Correspondence to Jinny Paul .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Cite this protocol

Paul, J. (2015). Blotting of Coomassie Blue-Stained Proteins from PAGE Gels to Transparencies. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_41

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_41

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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