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Grid-Immunoblotting

Part of the Methods in Molecular Biology book series (MIMB, volume 1312)

Abstract

Grid-immunoblotting is a fast, simple, and efficient method for simultaneously testing multiple allergens utilizing small amount of antibody.

Key words

Allergens Antibodies Nitrocellulose 

References

  1. 1.
    Reese G, Schmechel D, Ayuso R, Lehrer SB (2001) Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity. J Chromatogr B 756:151–156CrossRefGoogle Scholar
  2. 2.
    Yeliosof O, Scofield RH (2009) Grid-immunoblotting. Methods Mol Biol 536:289–292PubMedGoogle Scholar
  3. 3.
    Kurien BT, Scofield RH (2003) Protein blotting: a review. J Immunol Methods 274:1–15PubMedCrossRefGoogle Scholar
  4. 4.
    Demeulemester C, Peltre G, Laurente M, Panheleux D, David B (1987) Cyanogen bromide-activated nitrocellulose membranes: a new tool for immunoprint techniques. Electrophoresis 8:71CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Arthritis and Clinical Immunology ProgramOklahoma Medical Research FoundationOklahoma CityUSA
  2. 2.Department of MedicineUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  3. 3.Department of Veterans Affairs Medical CenterOklahoma CityUSA
  4. 4.Arthritis and Clinical Immunology Program, Oklahoma Medical Research FoundationUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

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