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Blotting from PhastGel to Membranes by Ultrasound

  • Protocol
Western Blotting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Ben Gurion University of the Negev, Beer-Sheva, 84105, Israel

    Joseph Kost & Aharon Azagury

Authors
  1. Joseph Kost
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  2. Aharon Azagury
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Corresponding author

Correspondence to Joseph Kost .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Kost, J., Azagury, A. (2015). Blotting from PhastGel to Membranes by Ultrasound. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_25

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_25

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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