Abstract
The history of the development of DNA blotting is described in this chapter. DNA blotting, involving the transfer of electrophoretically separated DNA fragments to a membrane support through capillary action, is also known as Southern blotting. This procedure enables the detection of a specific DNA sequence by hybridization with probes. The term Southern blotting led to a “geographic” naming tradition, with RNA blotting bearing the name Northern blotting and protein transfer to membranes becoming known as Western blotting.
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References
Ford PJ, Southern EM (1973) Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis. Nat New Biol 241:7–12
Kelly TJ, Smith HO (1970) A restriction enzyme from Hemophilus influenzae. II. J Mol Biol 51:393–409
Shinnick TM, Lund E, Smithies O, Blattner FR (1975) Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res 10:1911–1929
Chen CW, Thomas CA Jr (1980) A rapid two-dimensional fractionation of DNA restriction fragments. Anal Biochem 101:339–341
Gillespie D, Spiegelman S (1965) A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol 12:829–842
Sakonju S, Bogenhagen DF, Brown DD (1980) A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the region. Cell 19:13–25
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Southern, E. (2015). The Early Days of Blotting. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_1
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DOI: https://doi.org/10.1007/978-1-4939-2694-7_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2693-0
Online ISBN: 978-1-4939-2694-7
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