Blood Grouping Based on PCR Methods and Agarose Gel Electrophoresis

  • Ana Maria Sell
  • Jeane Eliete Laguila Visentainer
Part of the Methods in Molecular Biology book series (MIMB, volume 1310)

Abstract

The study of erythrocyte antigens continues to be an intense field of research, particularly after the development of molecular testing methods. More than 300 specificities have been described by the International Society for Blood Transfusion as belonging to 33 blood group systems. The polymerase chain reaction (PCR) is a central tool for red blood cells (RBC) genotyping. PCR and agarose gel electrophoresis are low cost, easy, and versatile in vitro methods for amplifying defined target DNA (RBC polymorphic region). Multiplex-PCR, AS-PCR (Specific Allele Polymerase Chain Reaction), and RFLP-PCR (Restriction Fragment Length Polymorphism-Polymerase Chain Reaction) techniques are usually to identify RBC polymorphisms. Furthermore, it is an easy methodology to implement. This chapter describes the PCR methodology and agarose gel electrophoresis to identify the polymorphisms of the Kell, Duffy, Kidd, and MNS blood group systems.

Key words

Blood group antigens Genotyping SNPs PCR Polymorphism 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ana Maria Sell
    • 1
  • Jeane Eliete Laguila Visentainer
    • 1
  1. 1.Laboratório de Imunogenética, Departamento de Ciências Básicas da SaúdeUniversidade Estadual de MaringáMaringáBrazil

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