Advertisement

Sequence-Based Typing for Platelet alloantigens

  • Shun-Chung Pai
  • Liang-In Lin
Part of the Methods in Molecular Biology book series (MIMB, volume 1310)

Abstract

Human platelet antigen (HPA) typing is largely performed by use of DNA-based techniques in patients that require assessing the risk of HPA alloimmunization. In this chapter, HPA typing by sequencing-based typing (SBT) techniques is described.

Key words

Human platelet antigen (HPA) Sequence-based typing (SBT) 

References

  1. 1.
    Deckmyn H, Ulrichts H, van de Walle G, Vanhoorelbeke K (2004) Platelet antigens and their function. Vox Sang 87(Suppl 2):105–111CrossRefPubMedGoogle Scholar
  2. 2.
    Pappalardo PA, Secord AR, Quitevis P, Haimowitz MD, Goldfinger D (2001) Platelet transfusion refractoriness associated with HPA-1a (Pl(A1)) alloantibody without coexistent HLA antibodies successfully treated with antigen-negative platelet transfusions. Transfusion 41:984–987CrossRefPubMedGoogle Scholar
  3. 3.
    Kroll H, Kiefel V, Santoso S (1998) Clinical aspects and typing of platelet alloantigens. Vox Sang 74(Suppl 2):345–354CrossRefPubMedGoogle Scholar
  4. 4.
    Curtis BR, McFarland JG (2014) Human platelet antigens - 2013. Vox Sang 106:93–102CrossRefPubMedGoogle Scholar
  5. 5.
    Tiller H, Killie MK, Chen P, Eksteen M, Husebekk A, Skogen B, Kjeldsen-Kragh J, Ni H (2012) Toward a prophylaxis against fetal and neonatal alloimmune thrombocytopenia: induction of antibody-mediated immune suppression and prevention of severe clinical complications in a murine model. Transfusion 52:1446–1457CrossRefPubMedGoogle Scholar
  6. 6.
    Metcalfe P, Watkins NA, Ouwehand WH, Kaplan C, Newman P, Kekomaki R, de Haas M, Aster R, Shibata Y, Smith J, Kiefel V, Santoso S (2003) Nomenclature of human platelet antigens. Vox Sang 85:240–245CrossRefPubMedGoogle Scholar
  7. 7.
    Curtis BR, McFarland JG (2009) Detection and identification of platelet antibodies and antigens in the clinical laboratory. Immunohematology 25:125–135PubMedGoogle Scholar
  8. 8.
    Hurd CM, Cavanagh G, Schuh A, Ouwehand WH, Metcalfe P (2002) Genotyping for platelet-specific antigens: techniques for the detection of single nucleotide polymorphisms. Vox Sang 83:1–12CrossRefPubMedGoogle Scholar
  9. 9.
    Skogen B, Bellissimo DB, Hessner MJ, Santoso S, Aster RH, Newman PJ, McFarland JG (1994) Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers. Transfusion 34:955–960CrossRefPubMedGoogle Scholar
  10. 10.
    Arinsburg SA, Shaz BH, Westhoff C, Cushing MM (2012) Determination of human platelet antigen typing by molecular methods: importance in diagnosis and early treatment of neonatal alloimmune thrombocytopenia. Am J Hematol 87:525–528CrossRefPubMedGoogle Scholar
  11. 11.
    Sachs UJ, Kiefel V, Kroll H, Bein G, Santoso S (2012) Report on the 15th International Society of Blood Transfusion platelet immunology workshop. Vox Sang 103:343–351CrossRefPubMedGoogle Scholar
  12. 12.
    Liu TC, Shih MC, Lin CL, Lin SF, Chen CM, Chang JG (2002) Gene frequencies of the HPA-1 to HPA-8w platelet antigen alleles in Taiwanese, Indonesian, and Thai. Ann Hematol 81:244–248CrossRefPubMedGoogle Scholar
  13. 13.
    Ficko T, Galvani V, Rupreht R, Dovc T, Rozman P (2004) Real-time PCR genotyping of human platelet alloantigens HPA-1, HPA-2, HPA-3 and HPA-5 is superior to the standard PCR-SSP method. Transfus Med 14:425–432CrossRefPubMedGoogle Scholar
  14. 14.
    Kengkate M, Butthep P, Kupatawintu P, Srisuddee A, Chantratita W, Hathalang O (2014) Comparison of a simple-probe real-time PCR and multiplex PCR techniques for HPA-1 to HPA-6 and HPA-15 genotyping. J Clin Lab Anal. doi: 10.1002/jcla.21734 PubMedGoogle Scholar
  15. 15.
    Shehata N, Denomme GA, Hannach B, Banning N, Freedman J (2011) Mass-scale high-throughput multiplex polymerase chain reaction for human platelet antigen single-nucleotide polymorphisms screening of apheresis platelet donors. Transfusion 51:2028–2033CrossRefPubMedGoogle Scholar
  16. 16.
    Bertrand G, Bianchi F, Chenet C, Martageix C, Blanchet P, Baumler M, Kaplan C (2006) New mutation in the platelet beta3-integrin gene: implication for the diagnosis of fetomaternal alloimmunization. Transfusion 46:2138–2141CrossRefPubMedGoogle Scholar
  17. 17.
    Skogen B, Wang R, McFarland JG, Newman PJ (1996) A dinucleotide deletion in exon 4 of the PlA2 allelic form of glycoprotein IIIa: implications for the correlation of serologic versus genotypic analysis of human platelet alloantigens. Blood 88:3831–3836PubMedGoogle Scholar
  18. 18.
    Smith GA, Rankin A, Riddle C, Cheetham-Wilkinson C, Ranasinghe E, Ouwehand WH, Watkins NA (2007) Severe fetomaternal alloimmune thrombocytopenia due to anti-human platelet antigen (HPA)-1a in a mother with a rare and silenced ITGB3*0101 (GPIIIa) allele. Vox Sang 93:325–330CrossRefPubMedGoogle Scholar
  19. 19.
    Wu GG, Tang QM, Shen WD, Liao Y, Li HC, Zhao TM (2008) DNA sequencing-based typing of HPA-1 to HPA-17w systems. Int J Hematol 88:268–271CrossRefPubMedGoogle Scholar
  20. 20.
    Xu X, Zhu F, Ying Y, Tao S, Liu Y, Hong X, Yan L (2009) Simultaneous genotyping of human platelet antigen-1 to 17w by polymerase chain reaction sequence-based typing. Vox Sang 97:330–337CrossRefPubMedGoogle Scholar
  21. 21.
    Pai S-C, Burnouf T, Chen J-W, Lin L-I (2013) Human platelet antigen alleles in 998 Taiwanese blood donors determined by sequence-specific primer polymerase chain reaction. Biomed Res Int 973789Google Scholar
  22. 22.
    Wang R, McFarland JG, Kekomaki R, Newman PJ (1993) Amino acid 489 is encoded by a mutational “hot spot” on the beta 3 integrin chain: the CA/TU human platelet alloantigen system. Blood 82:3386–3391PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Taiwan Blood Services FoundationTaipeiTaiwan
  2. 2.Department of Clinical Laboratory Sciences and Medical Biotechnology, College of MedicineNational Taiwan UniversityTaipeiTaiwan
  3. 3.Department of Laboratory MedicineNational Taiwan University HospitalTaipeiTaiwan

Personalised recommendations