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Extraction and Enrichment of Protein from Red and Green Macroalgae

  • Pádraigín A. Harnedy
  • Richard J. FitzGerald
Part of the Methods in Molecular Biology book series (MIMB, volume 1308)

Abstract

Macroalgae, in particular red and green species, are gaining interest as protein-rich foods for human consumption and sources of proteinaceous biofunctional peptide ingredients. During protein extraction the starting raw material, the cell disruption method utilized and the reagents employed have a major effect on the yield of protein recovered. A method is described herein for extraction and semi-purification of food-grade aqueous and alkaline soluble proteins from red and green macroalgae. Dried milled macroalgae are disrupted by osmotic shock with subsequent removal of aqueous soluble proteins by centrifugation. Alkaline soluble proteins are removed following consecutive treatment of the resultant pellet with an alkaline solution. Aqueous and alkaline soluble proteins are then enriched from the crude extracts by isoelectric precipitation.

Key words

Alkaline protein extracts Aqueous protein extract Cell disruption Chlorophyta Macroalgae Protein extraction Rhodophyta 

Notes

Acknowledgements

This research (Grant-Aid Agreement No. MFFRI/07/01) was carried out under the Sea Change Strategy with the support of the Marine Institute and the Department of Agriculture, Food and the Marine, funded under the National Development Plan 2007–2013.

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Copyright information

© Springer Science+Business Media, New York 2015

Authors and Affiliations

  • Pádraigín A. Harnedy
    • 1
  • Richard J. FitzGerald
    • 1
  1. 1.Department of Life SciencesUniversity of LimerickLimerickIreland

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