Abstract
This chapter describes three different spectroscopic methods for structural characterization of the commercial important hydrocolloid alginate extracted from brown seaweed. The “golden” reference method for characterization of the alginate structure is 1H liquid-state NMR of depolymerized alginate polymers using a stepwise hydrolysis. Having implemented this method, predictive and rapid non-destructive methods using vibrational spectroscopy and chemometrics can be developed. These methods can predict the M/G-ratio of the intact alginate powder with at least the same precision and accuracy as the reference method in a fraction of the time that is required to measure the alginate using the reference method. The chapter also demonstrates how solid-state 13C CP/MAS NMR can be used to determine the M/G ratio on the intact sample by the use of multivariate chemometrics and how this method shares the characteristics of the solid-state non-destructive IR method rather than its liquid-state counterpart.
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Acknowledgements
The work has been supported by a grant to Tina Salomonsen for an Industrial Ph.D. and by a grant from the strategic research council to the MicroPAT project under the InSPIRe (Danish Industry-Science Partnership for Innovation and Research in Food Science) consortium. The Faculty of Science and The Ministry of Science and Technology is acknowledged for a grant to the NMR metabolomics infrastructure.
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Jensen, H.M., Larsen, F.H., Engelsen, S.B. (2015). Characterization of Alginates by Nuclear Magnetic Resonance (NMR) and Vibrational Spectroscopy (IR, NIR, Raman) in Combination with Chemometrics. In: Stengel, D., Connan, S. (eds) Natural Products From Marine Algae. Methods in Molecular Biology, vol 1308. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2684-8_22
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DOI: https://doi.org/10.1007/978-1-4939-2684-8_22
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