Characterization of Phlorotannins from Brown Algae by LC-HRMS
Phlorotannins are a class of polyphenols found in brown seaweeds that have significant potential for use as therapeutics, owing to their wide range of bioactivities. Molecular characterization of phlorotannin-enriched extracts is challenging due to the extreme sample complexity and the wide range of molecular weights observed. Herein, we describe a method for characterizing phlorotannins employing ultrahigh-pressure liquid chromatography (UHPLC) operating in hydrophilic interaction liquid chromatography (HILIC) mode combined with high-resolution mass spectrometry (HRMS).
Key wordsBrown seaweed High-resolution mass spectrometry Hydrophilic interaction liquid chromatography Phlorotannins Polyphenols
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