Extraction, Isolation, and Identification of Sesquiterpenes from Laurencia Species

  • Angélica Ribeiro Soares
Part of the Methods in Molecular Biology book series (MIMB, volume 1308)


The knowledge about the chemical structure of the secondary metabolites and their relative abundances in algae is very important to several fields of basic and applied research in biology, chemistry, and many other disciplines. The attainment of such knowledge requires special attention to the origin of the organism in question and the methodology applied. Here, we present a protocol to obtain and identify some sesquiterpenes from Laurencia species based on traditional methodologies, such as flash and thin-layer chromatographies, nuclear magnetic resonance spectroscopy, and gas chromatography mass spectrometry. Red algae of the genus Laurencia are known to produce structurally diverse terpenes; most of them are halogenated compounds with important ecological functions and significant potential for the discovery of new biotechnological applications.

Key words

Chromatography fingerprints Flash chromatography GC–MS Halogenated sesquiterpenes NMR Sesquiterpenes Thin-layer chromatography 



This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and FINEP (3175/06). A.R. Soares is also grateful to CNPq for a research fellowship. The author thanks Dr. Heitor M. Duarte and Dr. Alessandra L. Valverde for valuable comments and corrections. The author also thanks Nathalia N.P. Carneiro and Fernanda L.S. Machado for some analyzes.


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Copyright information

© Springer Science+Business Media, New York 2015

Authors and Affiliations

  1. 1.Núcleo em Ecologia e Desenvolvimento Sócioambiental de Macaé, Grupo de Produtos Naturais de Organismos Aquáticos (GPNOA)Universidade Federal do Rio de JaneiroMacaéBrazil

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