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Plant Mitochondria pp 107–121Cite as

Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1305))

Abstract

The εN-acetylation of lysine side chains is a highly conserved posttranslational modification of both prokaryotic and eukaryotic proteins. Lysine acetylation not only occurs on histones in the nucleus but also on many mitochondrial proteins in plants and animals. As the transfer of the acetyl group to lysine eliminates its positive charge, lysine acetylation can affect the biological function of proteins. This chapter describes two methods for the identification of lysine-acetylated proteins in plant mitochondria using an anti-acetyllysine antibody. We describe the Western blot analysis of a two-dimensional blue native-polyacrylamide gel electrophoresis with an anti-acetyllysine antibody as well as the immuno-enrichment of lysine-acetylated peptides followed by liquid chromatography-tandem mass spectrometry data acquisition and analysis.

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Author notes

    Authors and Affiliations

    1. Plant Proteomics and Mass, Spectrometry Group, Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829, Cologne, Germany

      Markus Hartl, Ann-Christine König & Iris Finkemeier

    Authors
    1. Markus Hartl
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    2. Ann-Christine König
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    3. Iris Finkemeier
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    Corresponding author

    Correspondence to Iris Finkemeier .

    Editor information

    Editors and Affiliations

    1. La Trobe University, Bundoora, Victoria, Australia

      James Whelan

    2. The University of Western Australia, Crawley, West Australia, Australia

      Monika W. Murcha

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    Hartl, M., König, AC., Finkemeier, I. (2015). Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites. In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_7

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    • DOI: https://doi.org/10.1007/978-1-4939-2639-8_7

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-2638-1

    • Online ISBN: 978-1-4939-2639-8

    • eBook Packages: Springer Protocols

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