In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide
Superoxide (O2 −) and nitric oxide (NO) are produced within plant mitochondria and may have signaling functions within the cell. Here we describe semiquantitative fluorescence imaging-based approaches to estimate the mitochondrial amount of these reactive and short-lived species within intact leaf tissue. We also outline a biochemical method using oxyhemoglobin to measure NO within a whole leaf tissue extract. This quantitative method, while not specifically evaluating mitochondrial localized NO, does nonetheless provide another independent measure of NO that can be useful.
Key wordsDAF-FM Fluorescence confocal microscopy Leaf tissue Mitochondria MitoSOX Nitric oxide (NO) Oxyhemoglobin Superoxide (O2−)