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In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide

  • Marina Cvetkovska
  • Greg C. Vanlerberghe
Part of the Methods in Molecular Biology book series (MIMB, volume 1305)

Abstract

Superoxide (O2 ) and nitric oxide (NO) are produced within plant mitochondria and may have signaling functions within the cell. Here we describe semiquantitative fluorescence imaging-based approaches to estimate the mitochondrial amount of these reactive and short-lived species within intact leaf tissue. We also outline a biochemical method using oxyhemoglobin to measure NO within a whole leaf tissue extract. This quantitative method, while not specifically evaluating mitochondrial localized NO, does nonetheless provide another independent measure of NO that can be useful.

Key words

DAF-FM Fluorescence confocal microscopy Leaf tissue Mitochondria MitoSOX Nitric oxide (NO) Oxyhemoglobin Superoxide (O2

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity of Toronto ScarboroughTorontoCanada
  2. 2.Department of Cell and Systems BiologyUniversity of Toronto ScarboroughTorontoCanada

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