Abstract
The study of mitochondrial composition, biogenesis, and metabolism requires the isolation of intact functional mitochondria, often in relatively large quantities sufficient for downstream biochemical and proteomic analysis. Here we describe techniques optimized for the isolation of functional mitochondria from a variety of tissues from the model monocot and dicot species Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa).
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Douce R, Moore AL, Neuburger M (1977) Isolation and oxidative properties of intact mitochondria isolated from spinach leaves. Plant Physiol 60:625–628
Eubel H, Heazlewood JL, Millar AH (2007) Isolation and subfractionation of plant mitochondria for proteomic analysis. Methods Mol Biol 355:49–62
Millar AH, Liddell A, Leaver CJ (2007) Isolation and subfractionation of mitochondria from plants. Methods Cell Biol 80:65–90
Neuburger M, Journet EP, Bligny R, Carde JP, Douce R (1982) Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys 217:312–323
Taylor NL, Stroher E, Millar AH (2014) Arabidopsis organelle isolation and characterization. Methods Mol Biol 1062:551–572
Day DA, Neuburger M, Douce R (1985) Biochemical-characterization of chlorophyll-free mitochondria from pea leaves. Aust J Plant Physiol 12:219–228
Millar AH, Sweetlove LJ, Giege P, Leaver CJ (2001) Analysis of the Arabidopsis mitochondrial proteome. Plant Physiol 127:1711–1727
Howell KA, Millar AH, Whelan J (2006) Ordered assembly of mitochondria during rice germination begins with pro-mitochondrial structures rich in components of the protein import apparatus. Plant Mol Biol 60:201–223
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Murcha, M.W., Whelan, J. (2015). Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa . In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_1
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DOI: https://doi.org/10.1007/978-1-4939-2639-8_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2638-1
Online ISBN: 978-1-4939-2639-8
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