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Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1305))

Abstract

The study of mitochondrial composition, biogenesis, and metabolism requires the isolation of intact functional mitochondria, often in relatively large quantities sufficient for downstream biochemical and proteomic analysis. Here we describe techniques optimized for the isolation of functional mitochondria from a variety of tissues from the model monocot and dicot species Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa).

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Correspondence to Monika W. Murcha .

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Murcha, M.W., Whelan, J. (2015). Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa . In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2639-8_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2638-1

  • Online ISBN: 978-1-4939-2639-8

  • eBook Packages: Springer Protocols

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