Immunocapture-Multiplex RT-PCR for the Simultaneous Detection and Identification of Plant Viruses and Their Strains: Study Case, Potato Virus Y (PVY)

Part of the Methods in Molecular Biology book series (MIMB, volume 1302)


Immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) is a sensitive, reproducible, and robust method for the detection and identification of RNA viruses. The IC step provides a simple method to isolate virus particles from plant tissue, particularly when inhibitory substances are present, and thus enables subsequent use of RT-PCR amplification for large-scale virus testing and typing. The multiplex format of the PCR is often used for the detection and identification of multiple virus/strain simultaneously to save time, labor, and cost. Potato virus Y (PVY) is one of the most economically important viruses infecting potato worldwide. PVY exists as a complex of at least nine strains and many more unclassified recombinants that vary in their genome structures, phenotypes, and their economic importance. In the current chapter, a detailed protocol of an IC-based, multiplex RT-PCR assay for the detection and identification of various PVY strains is described.

Key words

Immunocapture Potato virus Y RT-PCR Strain Identification 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Plant Soil and Entomological SciencesUniversity of IdahoMoscowUSA

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