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Silicon Photonic Micro-Ring Resonators for Drug Screening and Kinetic Analysis

  • Muzammil Iqbal
  • Rufus W. Burlingame
  • Randy Romero
  • Annabel Wang
  • Tyler Grove
  • Martin A. GleesonEmail author
Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Genalyte has developed a turnkey silicon photonic chip sensing platform (Maverick™) for rapid detection of multiple biological analytes from a drop of sample. We present here the system applied to multiplex detection of antibodies in serum, the detection of receptor–ligand interactions, and the kinetic characterization of binding. The core of the technology is a silicon microchip, on the surface of which we pattern 128 microscopic ring resonators covered by a single microfluidic channel. The rings are individually functionalized to bind antigens for detecting serum analytes directly, antibodies for detecting immune response, or other biomarkers of interest in a sample that is pumped through the channel over the rings. The frequency of each ring’s optical resonance is exquisitely sensitive to the mass of bound analyte. A laser in the Maverick instrument interrogates the 128 rings almost simultaneously to quantify the presence of analytes. Currently, each assay is performed in quadruplicate, with 2 flow channels per chip, providing 15 multiplexed assays plus 1 control in each channel. Twelve chips are packaged into a consumable cartridge that can measure 24 samples. A 96-well plate with foil cover holds the reagents for all the tests, including 24 wells with a buffer solution, into which the operator loads the samples. The user needs only to collect a few drops of sample, transfer them to the sample well, insert the measurement cartridge and the well plate into the unit, and press start. The instrument performs all fluid operations internally, connecting dedicated probes from the measurement cartridge to the appropriate wells in the plate and pumping the sample and reagents through the microfluidic channels on the sensor chips as required by the assay protocol.

Key words

Drug screening Kinetics Protein interactions Photonic ring resonance 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Muzammil Iqbal
    • 1
  • Rufus W. Burlingame
    • 1
  • Randy Romero
    • 1
  • Annabel Wang
    • 1
  • Tyler Grove
    • 1
  • Martin A. Gleeson
    • 1
    Email author
  1. 1.Genalyte, Inc.San DiegoUSA

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