Abstract
Noninvasive real-time evaluation of living cell conditions and functions is increasingly sought after in life science. Surface plasmon resonance (SPR) sensors can sensitively detect refractive index changes on the surface of a sensor chip without any labeling in a real-time manner. In this chapter, we first review the principle of SPR sensors and the applications of SPR sensors for detection of living cell reactions. We then introduce the technique to isolate and fix basophils on an SPR sensor chip. Finally, we describe the method to visualize individual basophil activation by means of SPR imaging (SPRI) sensor and the potential of basophil activation test by SPRI for clinical diagnosis of type I allergy.
Key words
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Acknowledgments
We wish to thank the Analysis Center of Life Science, Hiroshima University, for the use of their facilities. This work was supported in part by Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) from Hiroshima Prefectural Institute of Industrial Science and Technology, Japan, Innovation Plaza Hiroshima of JST (Japan Science and Technology Corporation), Takeda Science Foundation, Grant-in-Aid for Scientific Research and Program for Promotion of Basic and Applied Researches for Innovation in Bio-oriented Industry, Science and technology research promotion program for agriculture, forestry, fisheries and food industry.
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Glossary
- CD
-
Cluster of differentiation
- DMSO
-
Dimethyl sulfoxide
- DNP
-
Dinitrophenol
- DSP
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Dithiobis(succinimidyl propionate)
- HSA
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Human serum albumin
- MACS
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Magnetic activated cell sorting
- PBMC
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Peripheral blood mononuclear cell
- PDMS
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Polydimethylsiloxane
- RA
-
Resonance angle
- RBL-2H3
-
Rat basophilic leukemia-2H3
- RI
-
Refractive index
- SPR
-
Surface plasmon resonance
- SPRI
-
Surface plasmon resonance imaging
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Yanase, Y., Hide, M. (2015). Surface Plasmon Resonance for Clinical Diagnosis of Type I Allergy. In: Fang, Y. (eds) Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2617-6_21
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DOI: https://doi.org/10.1007/978-1-4939-2617-6_21
Publisher Name: Humana Press, New York, NY
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