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Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening

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Book cover Label-Free Biosensor Methods in Drug Discovery

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Cellular assays using label-free Digital Holographic Microscopy (DHM) have been previously validated for cell viability assays in a drug screening context. Our automated DHM system allows performing fast and cost-effective screening assays for a wide range of applications for monitoring cell morphological changes and cell movements upon interaction with interfering compounds. In addition to these classic phenotypic assays, it has been demonstrated that target-based cellular assays can also be addressed by DHM for therapeutically relevant chloride channel receptors. Our DH-imaging (DHI) technology, potentially scalable for screening by imaging approaches in a high-throughput manner can also deliver highly informative data through long term experiments. Three examples of phenotypic screens are detailed in the present chapter: a label-free profiling approach, a cell proliferation assay, and methods for monitoring the activity of the GABAA chloride channel receptor.

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Acknowledgements

This work was supported by the CTI program (grant No. 12669.1 PFLS-LS). The authors thank the staff of Lyncée Tec SA for their technical support on the DHM imaging system, Sandra Borel and Nathalie Ballanfat from the BSF-EPFL for cell preparation and culture, Dr. Pascal Jourdain for electrophysiology experiments, and Dr. Marc Chambon for fruitful discussions and pertinent comments about the manuscript.

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    Authors and Affiliations

    1. Biomolecular Screening Facility, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), AAB 0 08 (Bâtiment AAB), Station 15, Lausanne, 1015, Switzerland

      Benjamin Rappaz, Fabien Kuttler, Billy Breton & Gerardo Turcatti

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    1. Benjamin Rappaz
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    2. Fabien Kuttler
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    3. Billy Breton
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    4. Gerardo Turcatti
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    Correspondence to Gerardo Turcatti .

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    Editors and Affiliations

    1. Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York, USA

      Ye Fang

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    Rappaz, B., Kuttler, F., Breton, B., Turcatti, G. (2015). Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening. In: Fang, Y. (eds) Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2617-6_17

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    • DOI: https://doi.org/10.1007/978-1-4939-2617-6_17

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-2616-9

    • Online ISBN: 978-1-4939-2617-6

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