Abstract
Surface plasmon resonance spectroscopy (SPR), a technique widely used for the study of biomolecular interactions, was recently demonstrated as a valuable label-free method to monitor live cell responses in real time. In contrast to molecular and biochemical assays, which rely on the detection of a unique marker such as cAMP, protein phosphorylation, or intracellular calcium, SPR is capable to detect and follow cell responses integrating the modulation of all signaling pathways. In recent studies, our group and others showed that SPR is a sensitive tool to study a broad range of cellular events on different timescales, ranging from rapid receptor-mediated responses (seconds to minutes) to long-lasting processes such as programmed cell death which typically occur over several hours. SPR allows the study of cellular responses evoked by different types of ligands through the analysis of parameters extracted from the SPR sensorgrams. For example, we previously showed that SPR allows the study of cellular responses evoked by the activation of G protein-coupled receptors (GPCRs) such as mu opioid receptor, protease-activated receptor-1, and angiotensin receptor type 1. Using pharmacological inhibitors it is possible to delineate the contribution of specific signaling pathways to the overall SPR response. Given the growing interest surrounding GPCR functional selectivity, this chapter describes how SPR spectroscopy can be used to study signaling cascades in living cells and, by way of consequence, functional selectivity.
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Bourassa, P., Söllradl, T., Maltais, JS., Charette, P.G., Gendron, L., Grandbois, M. (2015). Surface Plasmon Resonance to Study Cell Signaling and GPCR Functional Selectivity in Live Cells. In: Fang, Y. (eds) Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2617-6_10
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DOI: https://doi.org/10.1007/978-1-4939-2617-6_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2616-9
Online ISBN: 978-1-4939-2617-6
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