Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts

  • Sreejit Parameswaran
  • Rajalakshmi Santhakumar
  • Prasanna Vidyasekar
  • Rama S. Verma
Part of the Methods in Molecular Biology book series (MIMB, volume 1299)


In vitro culture of neonatal murine cardiomyocytes is vital for understanding the functions of the heart. Cardiomyocyte cultures are difficult to maintain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step.

Key words

Primary cell culture Neonatal mice Murine cardiomyocyte enrichment Immunocytochemistry 



This work is supported by grants to R.S.V. by the Ministry of Human Resource Development (MHRD—BIO/2005–2006/007/MHRD/RAMS/859) and Department of Biotechnology, Ministry of Science and Technology (DBT-BT/PR5392/MED/14/693/2004).


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sreejit Parameswaran
    • 1
  • Rajalakshmi Santhakumar
    • 1
  • Prasanna Vidyasekar
    • 1
  • Rama S. Verma
    • 1
  1. 1.Stem Cell and Molecular Biology Laboratory, Department of BiotechnologyIndian Institute of Technology MadrasChennaiIndia

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