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Cardiomyocytes pp 153-160 | Cite as

Cryopreservation of Neonatal Cardiomyocytes

  • Adam C. Vandergriff
  • M. Taylor Hensley
  • Ke Cheng
Part of the Methods in Molecular Biology book series (MIMB, volume 1299)

Abstract

Cardiomyocytes are frequently used for in vitro models for cardiac research. The isolation of cells is time-consuming and, due to the cells limited proliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and subsequently thawing them for use in assays.

Key words

Cardiomyocytes Cryopreservation Cardiac research 

Notes

Acknowledgement

This work was supported by funding from American Heart Association 12BGIA12040477, NC State University Chancellor’s Faculty Excellence Program, and National Natural Science Foundation of China H020381370216.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Adam C. Vandergriff
    • 1
    • 2
  • M. Taylor Hensley
    • 1
  • Ke Cheng
    • 2
    • 3
  1. 1.Department of Molecular Biomedical Sciences, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA
  2. 2.UNC/NCSU Joint Department of Biomedical EngineeringChapel HillUSA
  3. 3.Department of Molecular Biomedical Sciences, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA

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