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Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens

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RNA Nanotechnology and Therapeutics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1297))

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Abstract

Aptamers are oligonucleotides displaying specific binding properties for a predetermined target. They can be easily immobilized on various surfaces such as nanoparticles. Functionalized particles can then be used to various aims. We took advantage of the AlphaScreen® technology for monitoring aptamer-mediated interactions. A particle bearing an aptamer contains a photosensitizer whereas another type of particle contains a chemiluminescer. Irradiation causes the formation of singlet oxygen species in the photosensitizer-containing bead that in turn activates the chemiluminescer. Luminescence emission can be observed if the two types of beads are in close proximity (<200 nm). This is achieved when the cognate ligand of the aptamer is grafted onto the chemiluminescer-containing bead. Using this technology we have screened oligonucleotide libraries and monitored aptamer–protein interactions. This constitutes the basis for aptamer-based analytical assays.

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Acknowledgements

We are grateful to Pr D. Desmecht and Dr F. Cornet (University of Liège, Belgium) for help in identification of C1 and C6 aptamers and to T. Crosson for technical assistance.

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Authors and Affiliations

  1. Novaptech, European Institute of Chemistry and Biology, Pessac, France

    Laetitia Evadé & Emilie Daguerre

  2. University of Bordeaux, 146 rue Léo Saignat, 33076, Bordeaux, France

    Eric Dausse, Said Taouji, Eric Chevet & Jean-Jacques Toulmé

  3. Inserm U869, ARNA Laboratory, European Institute of Chemistry and Biology, Bordeaux, France

    Eric Dausse & Jean-Jacques Toulmé

  4. Inserm U1053, University of Bordeaux, Bordeaux, France

    Said Taouji & Eric Chevet

Authors
  1. Laetitia Evadé
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  2. Eric Dausse
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  3. Said Taouji
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  4. Emilie Daguerre
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  5. Eric Chevet
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  6. Jean-Jacques Toulmé
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Corresponding author

Correspondence to Jean-Jacques Toulmé .

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Editors and Affiliations

  1. Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical Sciences, University of Kentucky, Lexington, Kentucky, USA

    Peixuan Guo

  2. Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical Sciences, University of Kentucky, Lexington, Kentucky, USA

    Farzin Haque

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Evadé, L., Dausse, E., Taouji, S., Daguerre, E., Chevet, E., Toulmé, JJ. (2015). Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens. In: Guo, P., Haque, F. (eds) RNA Nanotechnology and Therapeutics. Methods in Molecular Biology, vol 1297. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2562-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2562-9_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2561-2

  • Online ISBN: 978-1-4939-2562-9

  • eBook Packages: Springer Protocols

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