Direct Cloning of Double-Stranded RNAs

  • Manli Shen
  • Marina Falaleeva
  • Natalia Korotkova
  • Stefan Stamm
Part of the Methods in Molecular Biology book series (MIMB, volume 1296)

Abstract

Most annotated genomes show a large number of sense–antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

Key words

Cloning dsRNA Sense–antisense pair 

Notes

Acknowledgment

This work was supported by GM083187 and P20GM1034865-10 from the NIH and an endowment of the University of Kentucky.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Manli Shen
    • 1
  • Marina Falaleeva
    • 1
  • Natalia Korotkova
    • 1
  • Stefan Stamm
    • 1
  1. 1.University of KentuckyLexingtonUSA

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