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The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads

  • Marisa M. FaraldoEmail author
  • Marina A. Glukhova
  • Marie-Ange Deugnier
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1293)

Abstract

The transplantation of mammary epithelial cells into the cleared fat pad allows their growth and differentiation in their normal physiological environment. This technique involves the grafting of tissue fragments or isolated cells into the mammary fat pads of prepubertal mice from which the endogenous epithelium has been surgically removed. Such transplantation assays are particularly useful for the analysis of morphogenetic potential and stem cell activity in normal mammary epithelium and breast tumors. We describe here the main steps in the transplantation of epithelial fragments and isolated cells from mouse mammary glands and the various approaches currently used to evaluate the regeneration and self-renewal properties of mammary stem cells.

Key words

Transplantation Outgrowth Mammary fat pad Mouse Stem cell 

Notes

Acknowledgements

This work was supported by La Ligue Nationale Contre le Cancer (Equipe Labelisée 2009, 2013) and a grant from Agence Nationale de la Recherche ANR-08-BLAN-0078-01 to MAG. MAG is Directeur de Recherche, MAD and MMF are Chargé de Recherche at the Institut National de la Santé et de la Recherche Médicale (INSERM).

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Copyright information

© Springer Science+Business Media LLC New York 2015

Authors and Affiliations

  • Marisa M. Faraldo
    • 1
    Email author
  • Marina A. Glukhova
    • 1
  • Marie-Ange Deugnier
    • 1
  1. 1.Centre de Recherche, UMR144, CNRSInstitut Curie, PSL Research UniversityParisFrance

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