The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads
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The transplantation of mammary epithelial cells into the cleared fat pad allows their growth and differentiation in their normal physiological environment. This technique involves the grafting of tissue fragments or isolated cells into the mammary fat pads of prepubertal mice from which the endogenous epithelium has been surgically removed. Such transplantation assays are particularly useful for the analysis of morphogenetic potential and stem cell activity in normal mammary epithelium and breast tumors. We describe here the main steps in the transplantation of epithelial fragments and isolated cells from mouse mammary glands and the various approaches currently used to evaluate the regeneration and self-renewal properties of mammary stem cells.
Key wordsTransplantation Outgrowth Mammary fat pad Mouse Stem cell
This work was supported by La Ligue Nationale Contre le Cancer (Equipe Labelisée 2009, 2013) and a grant from Agence Nationale de la Recherche ANR-08-BLAN-0078-01 to MAG. MAG is Directeur de Recherche, MAD and MMF are Chargé de Recherche at the Institut National de la Santé et de la Recherche Médicale (INSERM).
- 15.Dunphy KA, Tao L, Jerry DJ (2010) Mammary epithelial transplant procedure. J Vis Exp (40). doi: 10.3791/1849