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Single-Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing

  • Ana M. Aransay
  • Laura Barcena
  • Aintzane Gonzalez-Lahera
  • Nuria Macias-Camara
Part of the Methods in Molecular Biology book series (MIMB, volume 1293)

Abstract

Single-cell genome and transcriptome characterizations will probe to be decisive within the stem cells research, especially to describe appropriately the genetic impact of the diverse stem cells populations that are present in each organism. In the present chapter, we describe in detail how to prepare sequencing libraries out of single cells, for whole genome DNA and mRNA sequencing.

Key words

Single-cell genome Single-cell transcriptome Sequencing library High-throughput sequencing (HTS) 

Notes

Acknowledgements

We would like to thank Juan José Fuentes Nuño for his assistance on single-cell isolation procedure description. A.G.L. and some research expenses are partially supported by CIBERehd. A.M.A., N.M., A.G.L., L.B., and research expenses are supported by the Basque Country Government (Etortek Research Programs 2010/2014) and Bizkaia County’s Innovation Technology Department.

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Copyright information

© Springer Science+Business Media LLC New York 2015

Authors and Affiliations

  • Ana M. Aransay
    • 1
  • Laura Barcena
    • 2
  • Aintzane Gonzalez-Lahera
    • 1
  • Nuria Macias-Camara
    • 2
  1. 1.Genome Analysis PlatformCIC bioGUNE & CIBERehdDerioSpain
  2. 2.Genome Analysis PlatformCIC bioGUNEDerioSpain

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