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The side population (SP) assay has been utilized as a method for isolation and characterization of normal and cancer stem cells from a variety of tissues. However, the SP phenotype may not be a common property of all stem cells. This chapter reviews the principle and potential pitfalls of the SP assay with an emphasis on mammary gland SP cell analysis.
Key wordsSide Population (SP) Fluorescence-activated cell sorter (FACS) Stem cells Hoechst 33342 Breast cancer resistance protein (BCRP1/ABCG2)
The authors thank K99/R00 Pathway to Independence Award: 5R00CA127462-06 (F Behbod) and the Institute of Health Carlos III (PI11/02251), the Departments of Education (PI2009-7) and Health (2010-111060), and the Department of Industry, Tourism and Trade (Etortek) of the Government of the Autonomous Community of the Basque Country (MdM Vivanco) for financial support.