Side Population

  • Fariba BehbodEmail author
  • Maria d. M. Vivanco
Part of the Methods in Molecular Biology book series (MIMB, volume 1293)


The side population (SP) assay has been utilized as a method for isolation and characterization of normal and cancer stem cells from a variety of tissues. However, the SP phenotype may not be a common property of all stem cells. This chapter reviews the principle and potential pitfalls of the SP assay with an emphasis on mammary gland SP cell analysis.

Key words

Side Population (SP) Fluorescence-activated cell sorter (FACS) Stem cells Hoechst 33342 Breast cancer resistance protein (BCRP1/ABCG2) 



The authors thank K99/R00 Pathway to Independence Award: 5R00CA127462-06 (F Behbod) and the Institute of Health Carlos III (PI11/02251), the Departments of Education (PI2009-7) and Health (2010-111060), and the Department of Industry, Tourism and Trade (Etortek) of the Government of the Autonomous Community of the Basque Country (MdM Vivanco) for financial support.


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Copyright information

© Springer Science+Business Media LLC New York 2015

Authors and Affiliations

  1. 1.Pathology and Laboratory MedicineUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.CIC bioGUNETechnological Park of BizkaiaDerioSpain

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