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Humanization of the Mouse Mammary Gland

  • A. Wronski
  • L. M. Arendt
  • Charlotte KuperwasserEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1293)

Abstract

Although mouse models have provided invaluable information on the mechanisms of mammary gland development, anatomical and developmental differences between human and mice limit full understanding of this fundamental process. Humanization of the mouse mammary gland by injecting immortalized human breast stromal cells into the cleared murine mammary fat pad enables the growth and development of human mammary epithelial cells or tissue. This facilitates the characterization of human mammary gland development or tumorigenesis by utilizing the mouse mammary fat pad. Here we describe the process of isolating human mammary stromal and epithelial cells as well as their introduction into the mammary fat pads of immunocompromised mice.

Key words

Human-in-mouse model Humanization Human mammary epithelial cells Stroma Mammary gland Mammary gland biology 

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Copyright information

© Springer Science+Business Media LLC New York 2015

Authors and Affiliations

  • A. Wronski
    • 1
    • 2
  • L. M. Arendt
    • 1
    • 2
    • 3
  • Charlotte Kuperwasser
    • 1
    • 2
    Email author
  1. 1.Developmental, Molecular, and Chemical Biology Department, Sackler School of Graduate Biomedical SciencesTufts University School of MedicineBostonUSA
  2. 2.Raymond and Beverly Sackler Laboratory for the Convergence of Biomedical, Physical and Engineering SciencesMolecular Oncology Research Institute, Tufts Medical CenterBostonUSA
  3. 3.Department of Comparative BiosciencesSchool of Veterinary Medicine, University of Wisconsin-MadisonMadisonUSA

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