Abstract
Conventional cell culture plate based neuron–glia coculture methods have limitations in conducting localized physical and biochemical manipulations of cells that allow detailed investigation into axon–glia interactions and spatial regulation of functions. Here, we describe a multi-compartment neuron–glia coculture microsystem platform where axons can be fluidically and physically isolated from neuronal somata and dendrites so that interactions between the axon and glia can be studied in isolation. The multi-compartment configuration having six axon/glia compartments allows multiple experimental conditions to be conducted on a single microsystem.
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Acknowledgement
This work was supported by the National Institutes of Health/National Institute of Mental Health grant #1R21MH085267 and by the National Institutes of Health/National Institute of Neurological Disorders and Stroke grant #NS060017.
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Park, J., Kim, S., Li, J., Han, A. (2015). Multi-compartment Neuron–Glia Coculture Microsystem. In: Biffi, E. (eds) Microfluidic and Compartmentalized Platforms for Neurobiological Research. Neuromethods, vol 103. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2510-0_9
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DOI: https://doi.org/10.1007/978-1-4939-2510-0_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2509-4
Online ISBN: 978-1-4939-2510-0
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