Abstract
Limb regeneration studies have been extensively carried out in species of Ambystomatidae and Salamandridae families. So far limited research has been conducted in species belonging to the Plethodontidae family, where some of the species differs from other salamander families due to their direct development, thus absence of a larval life. Here, we describe a protocol to maintain the plethodontid salamanders of genus Bolitoglossa species under laboratory conditions to perform regeneration studies.
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Acknowledgments
Our work is funded by a program grant COLCIENCIAS 569 and Programa-Sostenibilidad 2013–2014 of the University of Antioquia. Claudia Arenas is funded by a fellowship from COLCIENCIAS 567. We thank Juan Manuel Daza for the taxonomic identification of the animals and Melisa Hincapie, Carlos Muñoz, Jose Fang, and Santiago Cuartas for their help during the field work and maintenance of the animals. The animals used to establish this protocol were collected under the environmental license “Permiso Marco de Recolección de Especímenes de Especies Silvestres de la Diversidad Biológica con Fines de Investigación” (Resolución #0524-27/05/2014). This work has the approval of the “Comité de Bioética para Experimentación con Animales” from the University of Antioquia.
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Arenas, C.M., Gómez-Molina, A., Delgado, J.P. (2015). Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies. In: Kumar, A., Simon, A. (eds) Salamanders in Regeneration Research. Methods in Molecular Biology, vol 1290. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2495-0_5
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DOI: https://doi.org/10.1007/978-1-4939-2495-0_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2494-3
Online ISBN: 978-1-4939-2495-0
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