Gene Manipulation for Regenerative Studies Using the Iberian Ribbed Newt, Pleurodeles waltl

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1290)

Abstract

Newts provide a unique model animal for regenerative studies. An experimental model system for molecular genetics in newts is needed in order to clarify the mechanisms of regeneration from the perspective of gene functions. We have identified that Iberian ribbed newt (Pleurodeles waltl) is a suitable model animal for such studies. Here we describe protocols for gene manipulation using Pleurodeles waltl.

Key words

Newt Salamander Molecular genetics Regeneration Transgenesis TALENs Genome editing 

Notes

Acknowledgments

We would like to thank Prof. Takashi Yamamoto and Dr. Tetsushi Sakuma (Hiroshima University) for providing the TALENs. We also thank Profs. Andras Simon and Anoop Kumar for their valuable help and advice. Kyorin Corporation (Hyogo, Japan) kindly provided the feed for the newts. This research was supported by MEXT/JSPS KAKENHI (grant numbers, 26650082, 25124706, and 25840086).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biomedical Sciences, School of Life ScienceFaculty of Medicine, Tottori UniversityYonagoJapan

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