Culture and Transfection of Axolotl Cells

  • Jean-François Denis
  • Fadi Sader
  • Patrizia Ferretti
  • Stéphane Roy
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1290)

Abstract

The use of cells grown in vitro has been instrumental for multiple aspects of biomedical research and especially molecular and cellular biology. The ability to grow cells from multicellular organisms like humans, squids, or salamanders is important to simplify the analyses and experimental designs to help understand the biology of these organisms. The advent of the first cell culture has allowed scientists to tease apart the cellular functions, and in many situations these experiments help understand what is happening in the whole organism. In this chapter, we describe techniques for the culture and genetic manipulation of an established cell line from axolotl, a species widely used for studying epimorphic regeneration.

Key words

Axolotl Cell culture Electroporation Limb regeneration Lipofection Salamander Transfection 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jean-François Denis
    • 1
  • Fadi Sader
    • 1
  • Patrizia Ferretti
    • 2
  • Stéphane Roy
    • 3
  1. 1.Department of Stomatology for Biochemistry, Faculty of Dentistry for MedicineUniversité de MontréalMontrealCanada
  2. 2.UCL Institute of Child HealthUniversity College LondonLondonUK
  3. 3.Department of Stomatology, Faculty of DentistryUniversité de MontréalMontrealCanada

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