Thyroxine-Induced Metamorphosis in the Axolotl (Ambystoma mexicanum)

  • Peggy S. Coots
  • Ashley W. Seifert
Part of the Methods in Molecular Biology book series (MIMB, volume 1290)


The axolotl (Ambystoma mexicanum) has remained an important model for regeneration and developmental biology for over a century. Although axolotls in captive-bred colonies usually exist in an aquatic form, they retain the ability to undergo metamorphosis following exposure to thyroid hormone. Here we present a robust method for inducing metamorphosis in adult axolotls that results in high survivability and produces terrestrial animals that can be maintained in long-term captivity.

Key words

Metamorphosis Thyroxine T4 Thyroid hormone Axolotl Ambystoma Salamander Regeneration 



This work was supported by the Office of the Vice President of Research at the University of Kentucky.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of BiologyUniversity of KentuckyLexingtonUSA

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