Histone Deacetylase Activity Assay

  • Lirong Peng
  • Zhigang Yuan
  • Edward SetoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1288)


Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ε-amino groups of conserved lysine residues in the amino terminal tail of histones. Accumulating evidence suggests that many, if not all, HDACs can also deacetylate nonhistone proteins. Through deacetylating histones and nonhistone proteins, HDACs regulate a variety of cellular processes including gene transcription, cell differentiation, DNA damage responses, and apoptosis. Aberrant HDACs are implicated in many human diseases and, therefore, it is important to have a consistent and reliable assay for analyzing HDAC activities. The focus of this chapter is to provide up-to-date, easy-to-follow, approaches and techniques, for the assay of HDAC enzymatic activities.

Key words

HDAC assay HDAC2 Sirtuin SIRT1 Protein deacetylation Core histone Boc-Lys(Ac)-AMC Radioactive assay Fluorometric assay 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Molecular Oncology DepartmentH. Lee Moffitt Cancer Center and Research InstituteTampaUSA
  2. 2.H. Lee Moffitt Cancer Center and Research InstituteTampaUSA

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