Preparation and Analysis of Positioned Mononucleosomes

  • Olga I. Kulaeva
  • Vasily M. StuditskyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1288)


Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.

Key words

Nucleosomes Chromatin Assembly Methods 



This work was supported by the NIH RO1 grant GM58650 and by the Russian Federal program grant 14.604.21.0063, RFMEFI60414X0063.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Cancer Epigenetics ProgramFox Chase Cancer CenterPhiladelphiaUSA
  2. 2.Biology FacultyLomonosov Moscow State UniversityMoscowRussia

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