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Non-radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length

  • Partha P. Banerjee
  • Shankar Jagadeesh
Part of the Methods in Molecular Biology book series (MIMB, volume 1288)

Abstract

The Telomeric repeat amplification protocol (TRAP) is a highly sensitive PCR-based assay and prove to be an important tool for understanding the role of telomerase in cancer and various tissues that harbors telomerase positive stem cells. This assay measures telomerase activity where the amount of target is dependent upon the activity of the enzyme. This protocol consists of two steps: first, telomeric repeats are added to the substrate by telomerase present in the cell and second, the extended products are amplified by Taq-DNA polymerase. The amplified TRAP products are separated on 10 % native PAGE and detected by SYBR Green I dye.

Key words

Telomerase TRAP assay Telomere SYBR Green I Southern blot 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular & Cellular BiologyGeorgetown University Medical CenterWashington, DCUSA

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