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Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support

  • Raphael SandaltzopoulosEmail author
  • Peter B. Becker
Part of the Methods in Molecular Biology book series (MIMB, volume 1288)

Abstract

Complex experimental strategies involving in vitro reconstituted chromatin or simple chromatin interaction studies are much facilitated by immobilizing the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This chapter details a convenient strategy for immobilization of linear plasmid DNA on streptavidin-coated beads, the reconstitution of chromatin on such beads, and some fundamental handling procedures.

Key words

Biotin-streptavidin Dynabeads Solid phase Chromatin assembly Chromatin constituents 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Molecular Biology and GeneticsDemocritus University of ThraceAlexandroupolisGreece
  2. 2.MolékularbiologieAdolf-Butenandt-Institut Ludwig-Maximilians-Universität München (LMU)MunichGermany

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