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Electroporation in the Rodent Retina In Vivo and In Vitro

  • Takahiko MatsudaEmail author
Protocol
Part of the Neuromethods book series (NM, volume 102)

Abstract

The retina is an excellent model system for studies of neural development and disease due to its simple structure and accessibility. We have been using an electroporation technique to analyze gene structure and function rapidly and conveniently in the mouse and rat retinas in vivo and in vitro (in retinal explants). By electroporation, various types of DNA constructs are readily introduced into the retina without DNA size limitation. In addition, more than two different DNA constructs can be introduced into the same cells at once with very high co-transfection efficiency. With this technique, we have established protocols for inducible gene misexpression and knockdown, as well as conventional gene misexpression and knockdown, in the retina. These methods will be useful to reveal the molecular mechanisms of retinal development and disease.

Key words

Retina Development Progenitors Photoreceptor cells Ganglion cells Electroporation Explant GFP 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institute for Integrated Cell-Material Sciences, Institute for Virus ResearchKyoto UniversitySakyo-kuJapan

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