Electroporation of Mycobacteria

  • Renan Goude
  • David M. Roberts
  • Tanya ParishEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1285)


High-efficiency transformation of DNA is integral to the study of mycobacteria, allowing genetic manipulation. Electroporation is the most widely used method for introducing DNA into mycobacterial strains. Many parameters contribute to high-efficiency transformation; these include the species per strain, the transforming DNA, the selectable marker, the growth medium additives, and the conditions of electroporation. In this chapter we provide an optimized method for the transformation of representative slow- and fast-growing species of mycobacteria—Mycobacterium tuberculosis and M. smegmatis, respectively.

Key words

Transforming DNA Selectable marker Electrocompetent cells Transformants 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.University of Rennes, Campus scientifique de BeaulieuRennesFrance
  2. 2.TB Discovery Research GroupInfectious Disease Research InstituteSeattleUSA

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