Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells Using Phenotypic Assays

  • Vincent Delorme
  • Ok-Ryul Song
  • Alain Baulard
  • Priscille BrodinEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1285)


Mycobacterium tuberculosis is able to colonize host cells, and it is now well admitted that the intracellular stage of the bacteria contributes to tuberculosis pathogenesis as well as to making it a persistent infection. There is still limited understanding on how the tubercle bacillus colonizes the cell and what are the factors impacting on its intracellular persistence. Recent advances in imaging technique allow rapid quantification of biological objects in complex environments. Furthermore, M. tuberculosis is a microorganism that is particularly genetically tractable and that tolerates the expression of heterologous fluorescent proteins. Thus, the intracellular distribution of M. tuberculosis expressing fluorescent proteins can be easily quantified by the use of confocal microscopy. Here we describe high-content/high-throughput imaging methods that enable tracking the bacillus inside host settings, taking into account the heterogeneity of colonization.

Key words

Mycobacterium tuberculosis High-content/high-throughput screening Chemogenomics Drug discovery siRNA library Automated confocal microscopy Image-based analysis 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Vincent Delorme
    • 1
  • Ok-Ryul Song
    • 1
  • Alain Baulard
    • 1
  • Priscille Brodin
    • 1
    Email author
  1. 1.Institut Pasteur de LilleUniversity of Lille Inserm U1019, CNRS UMR8204, Institut Pasteur de LilleLille CedexFrance

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